Journal: International Journal of Molecular Sciences

Article Title: High Adenosine Extracellular Levels Induce Glioblastoma Aggressive Traits Modulating the Mesenchymal Stromal Cell Secretome

doi: 10.3390/ijms21207706

Figure Lengend Snippet: Effects of ADO in the BM-MSC and GBM cell cross-talk. ( A ) The image depicts the contact-independent transwell cultures with BM-MSCs in the upper chamber and U343MG in the lower chamber. U343MG cell proliferation was evaluated after 24 and 48 h with Crystal violet, as reported in . ( B ) The image depicts the contact-independent transwell cultures with U343MG in the upper chamber and BM-MSCs in the lower chamber. BM-MSCs cell proliferation was evaluated after 48 h with Crystal violet, as reported in . ( C ) U343MG cells were seeded in the upper chamber and BM-MSCs treated with ADO (100 nM and 100 µM) in the lower chamber. After 24 h, U343MG cell invasion was analyzed using Matrigel basement membrane transwell system, as described in . Representative images are shown. Cell migration was quantified by counting the number of cells that migrated into the lower surface of the transwell membrane. The data are reported as percentage of cell invasion versus the CTRL set to 100% and are the means ± SEM of three independent experiments. The significance of the differences was determined by one-way ANOVA, followed by Bonferroni’s post-hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. CTRL.

Article Snippet: Human bone marrow MSCs (BM-MSCs) and glioblastoma stem cells (U343MG) were purchased by CLS (CLS Cell Lines Service GmbH, Eppelheim, Germany).

Techniques: Migration